Neo-glycoprotein Conjugates
Neo-glycoproteins and neo-glycopeptides, also known as neo-glycoconjugates, are synthetic glycoconjugates. They differ from naturally occurring glycoproteins in that they are not based on native linkages, and they may incorporate non-natural or altered glycan structures thereby creating novel molecules with specific properties. Neo-glycoproteins are chemically synthesized by reacting complementary functional groups on the glycan with those on the protein backbone. For example, glycans can be equipped with reactive handles such as activated esters or maleimides that can be chemo-selectively coupled to available amine groups on lysine residues or thiols on cysteines, respectively. Alternatively, glycans can be coupled to proteins using click chemistry approaches by reacting biorthogonal complementary functional groups such as azides and alkynes. Neo-glycoproteins exhibit diverse properties based on their glycan composition and valency compared to natural glycoproteins. They have been used for a variety of diagnostic and therapeutic applications including vaccine development, drug delivery, and glycan arrays.
We can provide neo-glycoproteins with a wide range of glycans (natural and non-natural) conjugated to proteins including bovine serum albumin (BSA), keyhole limpet hemocyanin (KLH), and others. The glycans can be coupled to the protein using a variety of chemistries including amine reactive, thiol reactive, and click-chemistry.
